Calpain-1, Human Erythrocyte

PRODUCT NUMBER:

MW: 110,000 Daltons
Catalytic Subunit = 80,000 Daltons
Regulatory Subunit = 30,000 Daltons

Frozen in 20 mM imidazole, 5mM β-mercaptoethanol, 1 mM EDTA, 1mM EGTA, 30% glycerol, pH 6.8

Storage: <= -70°C

Native Human Calpain-1 (u-Calpain) CAPN1, Human Erythrocyte direct from the manufacturer. Bulk qty. available.

Calpain-1 (μ-Calpain) is a calcium activated neutral cysteine protease. It is a heterodimer of a Catalytic 80 kDa subunit and a 30 kDa subunit, which requires 50 μM Ca2+ for half-maximal activity in vitro. Its participation in events such as cell division, signal transduction, and long term potentiation has been suggested. Calpains have also been proposed to play a role in various pathological processes associated with altered metabolism and altered calcium homeostasis.

Ref: Vilei, E.E. et al. 1997. Journal of Biological Chemistry. 272. 41

Activity: >= 1,000 units/mg protein.

One unit is defined as the amount of enzyme that will hydrolyze one ρmol Suc-LLVY-AMC per minute at 25°C using the Calpain Activity Assay Kit, Fluorogenic (EMD Cat. No. QIA120)

Purity: >= 95% by SDS-PAGE.

Prepared from whole blood shown to be non-reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.

Athens Research & Technology products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.