Immunoglobulin E (IgE), Normal Human Plasma

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MW: 200,000
Extinction Coefficient: 1.33

Frozen in 100 mM Tris, pH 7.5, with 200 mM NaCl and 0.05% sodium azide.

IgE is the least abundant immunoglobulin in plasma, found at a concentration of less that 0.6 micrograms/ml of normal plasma. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections. In a myeloma condition, IgE is produced by a single clone of plasma cells. The structure of myeloma IgE, however, is normal, and the immunoglobulin purified from a myeloma source is a useful protein for studying immunoglobulin behavior.

Storage: <= -20°C

Purity: >=95% by SDS-PAGE

Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.

Athens Research & Technology products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research use only.

Product Citations:
Janssen, Kris PF, et al. "Multiplexed protein detection using an affinity aptamer amplification assay." Analytical and bioanalytical chemistry 404, no. 6-7 (2012): 2073-2081.
Schachermeyer, Samantha, et al. "Aptamer-Protein Binding Detected by Asymmetric Flow Field Flow Fractionation."Journal of Chromatography A (2013).

Abe, Takaaki, et al. "Antibody against immunoglobulin E contained in blood components as causative factor for anaphylactic transfusion reactions." Transfusion (2014).

Kashiwakura, Jun-ichi, et al. "Most highly cytokinergic IgEs have polyreactivity to autoantigens." Allergy, asthma & immunology research 4, no. 6 (2012): 332-340.

Oh SS, et al. Synthetic aptamer-polymer hybrid constructs for programmed drug delivery into specific target cells.
J Am Chem Soc. 2014 Oct 22;136(42):15010-5. doi: 10.1021/ja5079464. Epub 2014 Oct 7.

Cao J, Wang H, Liu Y. Petal-like CdS nanospheres-based electrochemiluminescence aptasensor for detection of IgE with gold nanoparticles amplification. Spectrochim Acta A Mol Biomol Spectrosc. 2015;151:274-9. doi: 10.1016/j.saa.2015.06.104. Epub 2015 Jun 29.

Liu YM, et al. Aptamer-based detection and quantitative analysis of human immunoglobulin E in capillary electrophoresis with chemiluminescence detection. Electrophoresis. 2015 Oct;36(19):2413-8. doi: 10.1002/elps.201500158. Epub 2015 Jul 29.

Poongavanam MV, Kisley L, Kourentzi K, Landes CF, Willson RC.
Ensemble and single-molecule biophysical characterization of D17.4 DNA aptamer-IgE interactions. Biochim Biophys Acta. 2016 Jan;1864(1):154-64. doi: 10.1016/j.bbapap.2015.08.008. Epub 2015 Aug 22.

Garman L, Smith K, Muns EE, Velte CA, et al. Unique Inflammatory Mediators and Specific IgE Levels Distinguish Local from Systemic Reactions after Anthrax Vaccine Adsorbed Vaccination. Clin Vaccine Immunol. 2016 Aug 5;23(8):664-71. doi: 10.1128/CVI.00092-16. Print 2016 Aug.

Ref:
Ishiaka, K. 1985. Methods Enzymol. 116, 76.