Lipoproteins, High Density, Human Plasma

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Synonym: α-Lipoprotein, HDL, High density lipoprotein

MW: 175,000-360,000 Da

Liquid in 150 mM NaCl, pH 7.4, and 0.01% EDTA
Density: 1.063 to 1.210 g/ml; Composition: 55-45% lipid, 45-55% protein

Storage: 2-6 Degrees C; Do NOT Freeze; Store Away from Light

Shelf Life: minimum 6 months remaining

HDL is the vehicle for reversed cholesterol transport and estrification, serving as a scavenger for free cholesterol during intravascular catabolism of lipoproteins. HDL levels are inversely correlated with coronary heart disease. In a normal fasting individual, HDL concentrations range from 1.0-2.0 g/L.

Purity: Single arc by IEP against antisera to whole human serum. Essentially free of other plasma lipoproteins and chylomicrons as determined by electrophoresis using a SPIFE Vis Cholesterol gel kit for lipids and Coomassie Blue for proteins. >=95% of total lipoprotein content by electrophoresis.

Prepared from fresh, non-frozen plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Athens Research & Technology products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research use only.

Product Citations:
Matsuda, Akihiro, Kohjiro Nagao, Michinori Matsuo, Noriyuki Kioka, and Kazumitsu Ueda. "24 (S)‐hydroxycholesterol is actively eliminated from neuronal cells by ABCA1." Journal of neurochemistry (2013).

Homan, Reynold, Nadia Esmaeil, Laurel Mendelsohn, and Gregory J. Kato. "A fluorescence method to detect and quantitate sterol esterification by lecithin: cholesterol acyltransferase." Analytical biochemistry (2013).