Elastase, Human Neutrophil

100 ug
1 mg
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MW: 29,500
Extinction Coefficient: 0.985

Salt-free lyophilized solid.

Reconstitution: After centrifugation, we suggest addition of 50 mM Na Acetate, pH 5.5, with 150 mM NaCl to the original volume, followed by gentle mixing to ensure adequate homogenization.

Storage: <= -20°C

This enzyme degrades elastin, collagen, and proteoglycan and has been implicated in the development of pulmonary emphesema and rheumatoid arthritis.

Ref: Baugh, R.J. and Travis, J. 1976. Biochemistry. 15,836; Bode, W. et Al. 1986. EMBO J. 5 (10), 2453

Activity: 20-22 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one umole of MeO-Suc-Ala-Ala-Pro-Val-pNA per minute at 25oC, pH 7.5.

Purity: >=95% by SDS-PAGE

Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Athens Research & Technology products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research use only.

Product Citations:
Mittendorf, Elizabeth A., Gheath Alatrash, Na Qiao, Yun Wu, Pariya Sukhumalchandra, Lisa S. St John, Anne V. Philips et al. "Breast cancer cell uptake of the inflammatory mediator neutrophil elastase triggers an anticancer adaptive immune response." Cancer research 72, no. 13 (2012): 3153-3162

van den Berg, Carmen W., et al. "Mechanism of Neutrophil Dysfunction: Neutrophil Serine Proteases Cleave and Inactivate the C5a Receptor." The Journal of Immunology 192.4 (2014): 1787-1795.
Edwards, J. Vincent, Nicolette Prevost, Kandan Sethumadhavan, Abul Ullah, and Brian Condon. "Peptide conjugated cellulose nanocrystals with sensitive human neutrophil elastase sensor activity." Cellulose (2013): 1-13.

Valdez C, Wong YC, Schwake M, Bu G, Wszolek ZK, Krainc D.
Progranulin-mediated deficiency of cathepsin D results in FTD and NCL-like phenotypes in neurons derived from FTD patients.
Hum Mol Gen. 2017. 2017 Sep 25. doi:10.1093/hmg/ddx364

Kerros C, et al.
Neuropilin-1 mediates neutrophil elastase uptake and cross-presentation in breast cancer cells.
J Biol Chem. 2017 Jun 16;292(24):10295-10305. doi: 10.1074/jbc.M116.773051. Epub 2017 May 3.

Martin KR, Kantari-Mimoun C, Yin M, Pederzoli-Ribeil M, et al.
Proteinase 3 Is a Phosphatidylserine-binding Protein That Affects the Production and Function of Microvesicles.
J Biol Chem. 2016 May 13;291(20):10476-89. doi: 10.1074/jbc.M115.698639. Epub 2016 Mar 9.

Edwards JV, Fontenot KR, Prevost NT, Pircher N, Liebner F, Condon BD.
Preparation, Characterization and Activity of a Peptide-Cellulosic Aerogel Protease Sensor from Cotton.
Sensors (Basel). 2016 Oct 26;16(11). pii: E1789.

Chawla A, Alatrash G, Philips AV, Qiao N, et al.
Neutrophil elastase enhances antigen presentation by upregulating human leukocyte antigen class I expression on tumor cells.
Cancer Immunol Immunother. 2016 Jun;65(6):741-51. doi: 10.1007/s00262-016-1841-6. Epub 2016 Apr 29.

Sinden NJ, et al.
α-1-Antitrypsin variants and the proteinase/antiproteinase imbalance in chronic obstructive pulmonary disease.
Am J Physiol Lung Cell Mol Physiol. 2015 Jan 15;308(2):L179-90.

Ksiazek M, et al.
Miropin, a Novel Bacterial Serpin from the Periodontopathogen Tannerella forsythia, Inhibits a Broad Range of Proteases by Using Different Peptide Bonds within the Reactive Center Loop.
J Biol Chem. 2015 Jan 2;290(1):658-70. doi: 10.1074/jbc.M114.601716. Epub 2014 Nov 11.

Ramadass M, Ghebrehiwet B, Kew RR.
Enhanced recognition of plasma proteins in a non-native state by complement C3b. A possible clearance mechanism for damaged proteins in blood.
Mol Immunol. 2015 Mar;64(1):55-62. doi: 10.1016/j.molimm.2014.10.022. Epub 2014 Nov 15.

Thomas MP, et al.
Leukocyte Protease Binding to Nucleic Acids Promotes Nuclear Localization and Cleavage of Nucleic Acid Binding Proteins.
J Immunol. 2014 Jun 1;192(11):5390-7. doi: 10.4049/jimmunol.1303296. Epub 2014 Apr 25.

Ungurs MJ, Sinden NJ, Stockley RA.
Progranulin is a substrate for neutrophil-elastase and proteinase-3 in the airway and its concentration correlates with mediators of airway inflammation in COPD
Am J Physiol Lung Cell Mol Physiol. 2014 Jan 1;306(1):L80-7. doi: 10.1152/ajplung.00221.2013. Epub 2013 Nov 1.

Sinden NJ, Koura F, Stockley RA.
The significance of the F variant of alpha-1-antitrypsin and unique case report of a PiFF homozygote.
BMC Pulm Med. 2014 Aug 7;14:132. doi: 10.1186/1471-2466-14-132.

Kenninston JA, et al.
Inhibition of Plasma Kallikrein by a Highly Specific Active Site Blocking Antibody.
J Biol Chem. 2014 Aug 22;289(34):23596-608. doi: 10.1074/jbc.M114.569061. Epub 2014 Jun 26.

Tejera P, et al.
Functional Characterization of Polymorphisms in the Peptidase Inhibitor 3 (Elafin) Gene and Validation of Their Contribution to Risk of Acute Respiratory Distress Syndrome.
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Zheng K, et al.
Influence of glycosylation pattern on the molecular properties of monoclonal antibodies.
MAbs. 2014 May-Jun;6(3):649-58. doi: 10.4161/mabs.28588. Epub 2014 Mar 24.